Lab Report Tansport Across Membrane - 1672 Words

Biology Experiment 2 : Transport Across Membrane Name : Umi Adiba Anis Binti Che Hamid Matrix Number : CPM0147_2013B Batch : Pre-Med May Intake 2013 Group : E Date : 19.06.2013 Lecturer’s Name : Miss Nurul Izziani Objectives : I. To study the effects of hypotonic, hypertonic, and isotonic solutions on plant and animal cells. Experiment 1 : Introduction : The purpose of doing this experiment is to investigate the effects of hypotonic, hypertonic, and isotonic solutions on plant cell. Particularly in this experiment we use onion cells. Tissue from an onion is a good first exercise in using the microscope and viewing plant cells. The cells are easily visible under a microscope and the preparation of a thin section is†¦show more content†¦Material and methods : Materials | Apparatus | Potato Table salt/NaClâ‚‚Distilled water | Small knife Benchtop digital balance4 Vials Beakers (250 ml amp; 500 ml )Magnetic stirrer/stirring rodSpatula | I. Slice your potato into sticks that 4 – 6 cm long and 1 cm diameter. Record their initial weight (g) in 2 decimal places, e.g: 3.15 g. II. Prepare 4 vials with different salt solutions: 10% (w/v) NaCl, 3.5% (w/v) NaCl, 0.88% (w/v) NaCl, and distilled water. III. Put one potato stick into each solution. Record the weight of the potato sticks again after 20 min. Calculate the change in weight: final wt. – initial wt. IV. Calculate the percentage of water gain or loss as follow: V. % weight change = Final Wt – Initial Wt Ãâ€" 100 Initial Wt Results : Salt concentration | Initial weight of potato stick (g) | Final weight of potato stick (g) | Change in weight (g) | Change in weight (%) | Rank relative Water loss or gain | 10% NaCl | 0.88 | 0.76 | -0.12 | 13.6 | Loss | 3.5% NaCl | 0.88 | 0.83 | -0.05 | 5.68 | Loss | 0.88% NaCl | 0.88 | 0.88 | +0.00 | 0.00 | No change | Distilled Water | 0.88 | 0.97 | +0.09 | 10.2 | Gain | The data table for long stick potato Salt concentration | Initial